The main goal of this training is to offer to the attendees a broad introduction of the Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI), the most commonly used in MSI field. For this purpose, basics, principle, benefits, etc., will be addressed. In addition, lectures will also give an overview of the conventional workflow including sample preparation (cryo-sectioning, embedding, matrix deposition, etc.), acquisitions, quantification, as well as data processing based on SCiLS. The capacities of MALDI-MSI will be illustrated by concrete examples focused on biomedical research.